diff --git a/tests/e2e/specs/profile/username.test.js b/tests/e2e/specs/profile/username.test.js
new file mode 100644
index 000000000000..4ce1afcce03c
--- /dev/null
+++ b/tests/e2e/specs/profile/username.test.js
@@ -0,0 +1,31 @@
+import {
+	visitAdminPage,
+	createUser,
+} from '@wordpress/e2e-test-utils';
+
+describe( 'User Profile', () => {
+	it( 'Should not allow new user username as the email address of another profile', async () => {
+
+		// Creates a user `user1@example.com`.
+		await createUser('user1');
+
+		await visitAdminPage('user-new.php');
+		await page.waitForSelector('#user_login', {
+			visible: true
+		});
+		await page.$eval('#user_login', (el, value) => el.value = value, 'user1@example.com');
+		await page.$eval('#email', (el, value) => el.value = value, 'user2@example.com');
+
+		await page.click('#send_user_notification');
+
+		await Promise.all([page.click('#createusersub'), page.waitForNavigation({
+			waitUntil: 'networkidle0'
+		})]);
+
+		let adminNoticeErrors = await page.waitForSelector('.error');
+
+		expect(
+			await adminNoticeErrors.evaluate((element) => element.textContent)
+		).toContain('This username is not available. Please choose another one.');
+	} );
+} );